Psb1c3-stuffer
WebpSB1C3 (Search Vector Database) Vector type Mammalian Expression, CRISPR Growth in Bacteria Bacterial Resistance (s) Chloramphenicol, 25 μg/mL Growth Temperature 37°C Growth Strain (s) DH5alpha Copy number High Copy Gene/Insert Gene/Insert name eGFP Species Synthetic Promoter None Cloning Information Cloning method Restriction Enzyme WebThe ligations were transformed into strains DH5α and TOP10, but colonies were only obtained with the pSB1C3 vector. A repeated ligation into the vector containing the strong J23100 promoter gave two colonies. However, sequencing showed that while motA clones in pSB1C3 had the correct sequence, the two inserts downstream of BBa_J23100 had ...
Psb1c3-stuffer
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WebThe human immunodeficiency virus Tat-Binding Protein 1 (PSMC3, TBP1) is a 19S AAA-ATPase subunit that functions as a transcriptional activator (6-8). Research studies demonstrate that PSMC3/TBP1 may act as a tumor suppressor by promoting pVHL … WebUntil very recently, the main assembly standard supported by the iGEM registry was the BioBrick standard RFC[10]. However, iGEM Type IIS RFC[1000] assembly was recently accepted as a registry standard, and multiple Type IIS backbones and parts were present in the 2024 DNA distribution kit.We strongly believe in the efficiency and power of this …
WebIn the blue light starter system, we constructed the pSB1C3-stuffer-pro plasmid ( BBa_K4427009 ), which functions to synthesize EL222 protein upon induction of L-arabinose, and blue light irradiation induces EL222 protein dimerization onto the pBLind to initiate the expression of downstream red fluorescent protein (mRFP1). WebDownload pLKO.1-puro U6 sgRNA BfuAI stuffer.dna file. Download Plasmid Open in SnapGene. SnapGene. SnapGene is the easiest way to plan, visualize and document your everyday molecular biology procedures. Fast accurate construct design for all major …
WebImmunofluorescence analysis of PPP3CB was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room … WebEngineering a palette of eukaryotic chromoproteins for bacterial synthetic biology. Liljeruhm J, Funk SK, Tietscher S, Edlund AD, Jamal S, Wistrand-Yuen P, Dyrhage K, Gynna A, Ivermark K, Lovgren J, Tornblom V, Virtanen A, Lundin ER, Wistrand-Yuen E, Forster AC. J Biol Eng. 2024 May 10;12:8. doi: 10.1186/s13036-018-0100-0. eCollection 2024.
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WebFirstly, we tested whether our Psb1C3-stufffer-pro plasmid transformed properly in BL21 by CmR resistance screening, plasmid miniprep, XbaI and SpeI double digestion nucleic acid electrophoresis experiments, and the experimental results proved that we obtained the engineered bacterium BL21-Pro. down with desantisWebpSB1C3 includes also the antibiotic resistance of chloramphenicol. Chloramphenicol is a common lab antibiotic and does not degrade as quickly as ampicillin. It has a high-copy origin of replication which improves plasmid yield during extraction. cleaning fatty liverWebTools. Data Hub. Learn more about Addgene materials from user-contributed reports describing AAV and antibody experiments. Sequence Analyzer. Basic analysis for a user-entered sequence; includes restriction sites and map down with detroit t shirtsWebPlasmid pSB1C3-J23110-B0034-mRFP1E from Dr. Anthony Forster's lab contains the insert promoter, RBS, mRFP1 and is published in Anal Biochem. 2024 Sep 3:113936. doi: 10.1016/j.ab.2024.113936. This plasmid is available through Addgene. down with diabetesWeboligos, that were annealed and ligated into a pSB1C3 plasmid backbone. mtlR coding sequence was ordered as a gBlock Gene Fragment by IDT and inserted behind the R0040+B0032 promoter/RBS part in pSB1C3. This produced the regulatory plasmid BBa_K2442202 (Fig 2). The sequence of the p mtlE promoter with its native RBS was … down with disease bass tabWebBased on the synthetic biology pSB1C3 platform, the resulting vector offers a combination of useful features: the strong T7 promoter combined with lac operator, OmpA signal sequence, a selection of cloning sites located at convenient positions and … cleaning fatty liver naturallyWebSep 7, 2008 · Description. This is a high copy plasmid for use in assembly of BioBrick® standard biological parts. cleaning faucet aerators help flow of faucet