Hcrt-cre
WebJun 28, 2024 · Taking the LHA-BF neural circuit in Hcrt-Cre mice as an example, the opsin is typically introduced to the LHA neurons by injecting a virus containing ChR2 (A) or NpHR (B). After 3 weeks to allow for expression of the opsin, LHA-BF axon terminals can be targeted with corresponding light pulses from the optical fiber to excite ( A ) or inhibit ... WebNov 27, 2024 · Hcrt deficient mice lacking one copy of the 5HTT gene ... DR neurons of mice harboring loxP site-flanked alleles of both HCRT receptors 1&2 genes 27 with AAV expressing mCherry and Cre recombinase.
Hcrt-cre
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WebJun 16, 2024 · (A–C), Sigh rate of anesthetized adult Vglut2-IRES-Cre (A), Vgat-IRES-Cre (B), and Hcrt-IRES-Cre (C) knockin mice (mean ± SEM, n = 6 mice per condition) stereotactically injected with AAV-DIO-ChR2 into LHA, then photoactivated 2 weeks later with a blue laser for 4 min at 5 Hz (A and C) or 10 Hz (B) to optogenetically activate the … Web7z¼¯' êQ "fz H±¨BSDIFF40Z= Á _Òs BZh91AY&SYJ°è¥\×ÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿÿà2 {¸ CB Ø{ÞU÷Ûï¥Û ...
WebSelecting multiple terms will filter for results with any (i.e. logical OR) of the selected terms. WebMethods: Hcrt cre rats were generated using a CRISPR/Cas9-based approach. Viruses encoding optogenetic probes were injected into the perifornical lateral hypothalamic …
WebImages for hypocretin ( Hcrt ) TRAP (translating ribosome affinity purification) founder line JD218 This line is available from The Jackson Laboratory, stock ID 028619 Click on a thumbnail of mouse brain to examine the image in detail. WebJan 1, 2024 · Hcrt-cre rats were killed in the fifth week after virus injection for confirmation of virus expression and fibre location, immediately after completion of photostimulation …
Web摘要： 目的:研究小鼠下丘脑外侧区(LHA)内食欲素(orexin)神经元向腹侧被盖区(VTA)的投射及VTA靶神经元的类型及其所占比例.方法:利用脑立体定向技术将逆向示踪病毒AAVretro-DIO-mCherry注射至Hcrt-Cre小鼠的VTA,待病毒表达之后制备包含LHA的间脑切片,用免疫荧光染色法进行orexin阳性神经元染色,计算病毒表达 ...
WebOrexin receptors and dopaminergic neurones in the VTA were identified using immunofluorescence. Orexinergic terminals in the VTA were optogenetically regulated to detect the endogenous orexin-mediated regulation of dopaminergic neurones during anaesthesia in Hcrt cre rats. stimulus check for college students 2021WebJun 9, 2024 · C57BL/6J mice (4–6 months old) expressing GFP (Hcrt-GFP) (18, 38, 39, 44) or Cre-recombinase (Hcrt-Cre) selectively in hypocretin neurons under the control of a specific hypocretin promoter were used in this study. Mice were group housed and maintained on a 12-hour light/12-hour dark cycle (lights on at 6:00 am) with free access … stimulus check for divorced parentsWebLentiviral vectors contained either Hcrt::ChR2-mCherry or Hcrt::mCherry sequences and were prepared as previously described . Cre-inducible recombinant AAV vectors carrying optogenetic transgenes were serotyped with AAV5 coat proteins and packaged by the viral vector core at the University of North Carolina. stimulus check for elderly peopleWebSynergistic activation of CRH PVN and Hcrt LH neurons during restraint stress is consistent with our 155 observation of cFos staining as described in Fig. S1. 156 157 Mild optogenetic stimulation ... stimulus check for ctWebHcrt neurons were labeled by reporter virus injection into yound and aged Hcrt:cre mice. Specifically, 0.3 μl AAV vectors carrying genes encoding Cre-dependent DsRed- hTRF2 (AAV-DJ-DIO-DsRed-hTRF2, 1.95 × 1012 genome copies per ml, customer virus packaged at Stanford Virus Core) were bilaterally injected to the LH (AP: −1.35 mm, ML: ±0.95 ... stimulus check for farm workershttp://www.gensat.org/TRAPFounderView.jsp?founder_id=93983&gene_id=36 stimulus check for gasWebMar 14, 2024 · Hcrt-IRES-Cre mice were acquired by from Nanmo creatures. The study has been carried out in accordance with the governmental regulations on the use of experimental animals with prior approval by Laboratory Animal Ethical and Welfare Committee of Laboratory Animal Center of Ningxia Medical University (Document # … stimulus check for gas prices