Dna pk inhibitor nu7441
WebAug 31, 2024 · Two lead therapies, regorafenib (Reg) and NU7441, were selected based on their ability to alter a variety of immunomodulatory proteins, including CD55, CD73, CD155, programmed death-ligand 1 (PD-L1), nerve growth factor receptor (NGFR), and HLA class I in a heterogeneous panel of melanomas. WebMay 23, 2011 · NU7441 was identified as a potent and specific ATP competitive inhibitor of DNA-PK (IC 50 14 nmol/L) with 100-fold selectivity for DNA-PK relative to other members of the PI3KK family. NU7441 increased the sensitivity of colon cancer cells to IR and topoisomerase II inhibitors, and altered their cell cycle profile.
Dna pk inhibitor nu7441
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WebAug 27, 2015 · We find that NU7441 and KU-0060648 reduce the frequency of NHEJ while increasing the rate of HDR following Cas9-mediated DNA cleavage. Conclusions: Our results identify two small molecules compatible for use with Cas9-editing technology to improve the frequency of HDR. Publication types Research Support, Non-U.S. Gov't WebThe DNA-dependent protein kinase (DNA-PK) complex plays a pivotal role in non-homologous end-joining (NHEJ) repair. We investigated the mechanism of NU7441, a …
WebNU 7441 is a potent and selective DNA-PK inhibitor (IC 50 = 14 nM). Selective for DNA-PK over a range of kinases including mTOR, PI 3-K, ATM and ATR. Potentiates the effects … WebDNA-PK in human malignant disorders: Mechanisms and implications for pharmacological interventions
WebMay 29, 2012 · ATM kinase inhibitor KU55933 and DNA-PK kinase inhibitor NU7441 were reconstituted in DMSO and used at 10 μM and 5 μM, respectively . I-PpoI was induced in TPC-1 cells infected with HA-ER-I-PpoI retrovirus by using 4-OHT for 6 h in the presence of vehicle, KU55933, or NU7441. WebBAY-8400 (BAY8400) is a highly potent, selective inhibitor of DNA-PK with biochemical IC50 of 81 nM, cellular IC50 of 69 nM (γH2AX in HT-144). BAY-8400 showed good selectivity against PI3K and ATR, ATM and mTOR. In the KINOMEscan panel, >50% inhibition activity of BAY-8400 at 1 uM was measured only for three out of 365 kinases, …
WebMar 23, 2024 · PMID: 36373232 Regulation of DNA‐PKactivity promotes the progression of TNBCvia enhancing the immunosuppressive function of myeloid‐derived suppressor cells Jiawen Han,1 ,2 Minjie Wan,1 ,3 Zhanchuan Ma,1 ,2 and Huanfa Yi1 ,2 Jiawen Han 1 Central Laboratory, The First Hospital of Jilin University, Changchun China 2
WebNU7441 caused doxorubicin- and IR-induced DNA DSBs (measured by γ-H2AX foci) to persist and also slightly decreased homologous recombination activity, as assessed by Rad51 foci. Chemo- and radio-potentiation were induced by NU7441 in M059-Fus-1, but not in DNA-PK-deficient M059 J cells. hub international tpaWebMar 2, 2024 · If necessary, cells were treated with the DNA-PKcs inhibitor NU7441 (TopScience Cat# T6276) or Nocodazole (Sigma-Aldrich Cat# M1404) at 6 h post-transfection. NU7441 was replaced with a fresh addition of the drug the next day. Nocodazole was withdrawn after 12 h and replaced by fresh medium for the rest of the … hohe lottogewinneWebBiological Activity. NU 7441 is a potent and selective DNA-PK inhibitor (IC 50 = 14 nM). Selective for DNA-PK over a range of kinases including mTOR, PI 3-K, ATM and ATR. … hohe loog hütte anfahrtWebAccumulating evidence suggests a synergistic effect in cells simultaneously exposed to different types of clustered and dispersed DNA damage. We aimed to analyse the effect of mixed beams of alpha particles and X-rays (1:1 dose of each) on DNA damage response genes in human peripheral blood lymphocytes isolated from four donors. Two donors … hub international tnWebNU7441 is a selective inhibitor of DNA-dependent protein kinase (DNA-PK) with IC50 value of 13 nM [1]. NU7441 is an ATP-competitive inhibitor of DNA-PK and showed a Ki value of 0.65 nM. The inhibition of DNA-PK was selective. NU7441 showed no inhibition effect on the DNA-PK-related enzymes ATM and ATR at concentration of 100 μM. hub international texas inc el pasoWebDNA-PK and ATM are members of the phosphatidylinositol 3′-kinase like kinase (PIKK) family of serine/threonine protein kinases and have critical roles in the cellular response to DNA double-strand breaks. Genetic loss of either activity leads to pronounced sensitivity to ionizing radiation (IR). Hence, these enzymes are potential targets to confer enhanced … hub international texas inc dallas texasWebNU7441 (KU 57788) is a potent and selective DNA-dependent protein kinase (DNA-PK) inhibitor with IC50 values of 14, 1700, 5000, >100000 and >100000 nM for DNA-PK, mTOR, PI 3-K, ATM and ATR respectively. Buy PI3K inhibitor NU7441 (KU 57788) from AbMole BioScience. hohelith-cuxhaven